Coding

Part:BBa_K2309022:Design

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-26)


LL-37 with A His-Tag for Lactococcus lactis NZ9000 (codon optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The purpose of coupling 6xHis-tag with LL-37can also be used for purifying the anti-microbial peptide produced in the bacterial cells.6xHis tag is six histidine amino acids tandem arranged together which has a strong affinity with the nickel ion in the Ni-NTA purification system. After purification, immunodot blot method was used to confirm that LL-37 was produced.

Source

Homo sapiens

References